The O's take a break from league action tomorrow (3pm KO) when they welcome Beaconsfield Town to the Breyer Group Stadium.
The National League leaders fell to only their second league defeat of the season last week as a 4th minute strike by Femi Ilesanmi saw a 1-0 defeat for the O's.
The O's reached the FA Trophy Fourth Round last season, beating Haringey Borough, Bognor Regis Town and Dover before a defeat to Gateshead stopped them reaching the Semi-Final.
Gary Meakin's side currently sit in 7th position in the Evo-Stik Southern Premier South, having been promoted from the Southern Football League East Division last season.
They qualified for the First Round after defeating Leiston 3-1 with goals coming from Charlie Losasso, Jordan Ajanlekoko and Marvin Morgan.
The Rams secured a draw against Poole Town in their most recent fixture, Charlie Losasso's goal just before half-time earnng a point.
The O's are expected to make a couple of changes as Myles Judd will definitely miss the game while James Dayton is now a long-term absentee. Youngsters Arthur Janata and Hector Kyprianou cannot feature having been cup-tied while on loan while Charlie Lee may return to contention.
Will Finnie will be the referee for tomorrow's clash, assisted by Paul Evans and Bradley Alexander. Finnie refereed the O's FA Cup defeat to Maidstone United in October.
Can’t make the game? Follow live updates through the official Twitter feed, or listen to audio commentary from Dave Victor on Ozone.
Overseas supporters can access a live-stream for just £5 via this link.
Audio commentary will be available through the match centre which will appear at the top of the leytonorient.com homepage, with commentary starting 30 minutes before kick-off.
Match report and reaction following the game will appear on leytonorient.com.